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recombinant human mouse rat activin a  (R&D Systems)


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    R&D Systems recombinant human mouse rat activin a
    Recombinant Human Mouse Rat Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+mouse+rat+activin+a/Recombinant+Human%2FMouse%2FRat+Activin+A+Protein/pmc13120857-25-22-27
    Average 96 stars, based on 605 article reviews
    recombinant human mouse rat activin a - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.
    Article Snippet: • DAPI (Sigma-Aldrich, cat. no. D9542) ▲ CAutIon Because DAPI is photosensitive, exposure to light sources should be minimized. .. • B-27 supplement (50×), serum-free (Thermo Scientific, cat. no. 17504044) • l-Glutamine (200 mM) (Thermo Fisher Scientific, cat. no. 25030081) • MEM non-essential amino acids solution (100×) (NEAA; Thermo Scientific, cat. no. 11140050) • Penicillin-streptomycin (10,000 U/ml) (pen-strep; Thermo Fisher Scientific, cat. no. 15140122) • Recombinant human activin A (Biochemistry Department, University of Cambridge) or recombinant human/mouse/rat activin A (R&D Systems, cat. no. 338-AC) • GSK3βi CHIR99021 (Tocris, cat. no. 4423) • ALK2/3 inhibitor LDN193189 (Sigma-Aldrich, cat. no. SML0559) • Recombinant human fibroblast growth factor 4 (FGF4; R&D Systems, cat. no. 235-F4-025) • Corning Matrigel matrix, phenol red free, growth factor reduced (Matrigel; Corning, cat. no. 356231) Nature Protocols 8 .. • Paraformaldehyde solution (4% (wt/vol)), buffered, pH 6.9 (PFA; EMD Millipore, cat. no. 1.00496.0700) ▲ CAutIon 4% (wt/vol) PFA is a toxic and mutagenic reagent.

    Article Title: Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
    Article Snippet: Generation and maintenance of murine iBCs Cell differentiation was performed in a complete serum free differentiation medium (cSFDM) consisting of a base medium of IMDM (ThermoFisher, 12440053) and Ham’s F12 (Cellgro, 10-080-CV) with B27 Supplement with retinoic acid (Invitrogen, 17504-44), N2 Supplement (Invitrogen, 17502-048), 0.1% bovine serum albumin Fraction V (Life Technologies, 15260-037), monothioglycerol (Sigma, M6145), Glutamax (Life Techologies, 35050-061), ascorbic acid (Sigma, A4544), and Primocin (Invivogen, ANTPM1). .. Directed differentiation was performed by adapting our previously described approach for deriving airway organoids from mouse PSC.32,35,36 Briefly, to induce definitive endoderm fate, EBs were dissociated into a single cell suspension and cultured in cSFDM supplemented with 50ng/mL recombinant human/mouse/rat Activin A (R&D systems, 338-AC) for 60hr allowing EBs to reform in suspension culture. .. Next, 100ng/mL recombinant mouse Noggin (R&D systems, 1967-NG) and 10mM SB431542 (Sigma, S4317) were applied for 24hr to generate anterior foregut endoderm.

    Article Title: Production method for nerve tissue
    Article Snippet: .. For example, Recombinant Human/Mouse/Rat Activin A (R&D systems, #338-AC) is added to a concentration of about 1 ng/ml to about 10 μg/ml, preferably about 10 ng/ml to about 1 μg/ml, more preferably about 100 ng/ml. ..

    Article Title: Effects of increasing intranuclear calcium levels via MCU inhibition on mouse and human PSC-derived cardiomyocyte differentiation and maturation
    Article Snippet: Then, 1.5 × 10 6 hiPSCs were seeded on Matrigel (354277)-coated 35-mm dishes, grown on mTeSR1 (#85851; STEMCELL Technologies) until reaching 100% confluency, and subjected to cardiac differentiation. .. The following chemicals were sequentially administered: 6 μ m CHIR99021 (252917-06-9; Cayman) for the first 2 days, followed by 10 μ m recombinant human/mouse/rat activin A (338-AC; R&D Systems) and 20 μ m recombinant human bFGF (13256029; Thermo Fisher Scientific) the next day, and 5 μ m IWR1 (I0161; Sigma-Aldrich) 3 days after that. .. The medium was replaced with the Roswell Park Memorial Institute-1640 medium (11875-085; Thermo Fisher Scientific) supplemented with B27 (minus insulin) once every 2 days until the CMs contracted ( ).

    Article Title: Activin A, a Novel Chemokine, Induces Mouse NK Cell Migration via AKT and Calcium Signaling
    Article Snippet: .. Recombinant human/mouse/rat activin A (Cat# 338-AC), anti-human/mouse/rat activin A beta A subunit antibody (Cat# AF338) and enzyme-linked immunosorbent assay (ELISA) kit for activin A (Cat# DAC00B) were provided by R&D systems (Minneapolis, MN, USA). .. Recombinant murine CXCL12 (SDF-1α, Cat# 250-20A) and recombinant murine IL-2 (Cat# 212-12) were obtained from PeproTech (Rocky Hill, NJ, USA).

    Article Title: Production method for retinal tissue
    Article Snippet: .. For example, Recombinant Human/Mouse/Rat Activin A (R&D systems, #338-AC) is added to a concentration of about 1 ng/ml to about 10 μg/ml, preferably about 10 ng/ml to about 1 μg/ml, more preferably about 100 ng/ml. ..

    Article Title: Method for producing retinal tissue
    Article Snippet: .. For example, Recombinant Human/Mouse/Rat Activin A (R&D systems, #338-AC) is added to a concentration of about 1 ng/ml to about 10 μg/ml, preferably about 10 ng/ml to about 1 μg/ml, more preferably about 100 ng/ml. ..

    Single Cell:

    Article Title: Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
    Article Snippet: Generation and maintenance of murine iBCs Cell differentiation was performed in a complete serum free differentiation medium (cSFDM) consisting of a base medium of IMDM (ThermoFisher, 12440053) and Ham’s F12 (Cellgro, 10-080-CV) with B27 Supplement with retinoic acid (Invitrogen, 17504-44), N2 Supplement (Invitrogen, 17502-048), 0.1% bovine serum albumin Fraction V (Life Technologies, 15260-037), monothioglycerol (Sigma, M6145), Glutamax (Life Techologies, 35050-061), ascorbic acid (Sigma, A4544), and Primocin (Invivogen, ANTPM1). .. Directed differentiation was performed by adapting our previously described approach for deriving airway organoids from mouse PSC.32,35,36 Briefly, to induce definitive endoderm fate, EBs were dissociated into a single cell suspension and cultured in cSFDM supplemented with 50ng/mL recombinant human/mouse/rat Activin A (R&D systems, 338-AC) for 60hr allowing EBs to reform in suspension culture. .. Next, 100ng/mL recombinant mouse Noggin (R&D systems, 1967-NG) and 10mM SB431542 (Sigma, S4317) were applied for 24hr to generate anterior foregut endoderm.

    Suspension:

    Article Title: Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
    Article Snippet: Generation and maintenance of murine iBCs Cell differentiation was performed in a complete serum free differentiation medium (cSFDM) consisting of a base medium of IMDM (ThermoFisher, 12440053) and Ham’s F12 (Cellgro, 10-080-CV) with B27 Supplement with retinoic acid (Invitrogen, 17504-44), N2 Supplement (Invitrogen, 17502-048), 0.1% bovine serum albumin Fraction V (Life Technologies, 15260-037), monothioglycerol (Sigma, M6145), Glutamax (Life Techologies, 35050-061), ascorbic acid (Sigma, A4544), and Primocin (Invivogen, ANTPM1). .. Directed differentiation was performed by adapting our previously described approach for deriving airway organoids from mouse PSC.32,35,36 Briefly, to induce definitive endoderm fate, EBs were dissociated into a single cell suspension and cultured in cSFDM supplemented with 50ng/mL recombinant human/mouse/rat Activin A (R&D systems, 338-AC) for 60hr allowing EBs to reform in suspension culture. .. Next, 100ng/mL recombinant mouse Noggin (R&D systems, 1967-NG) and 10mM SB431542 (Sigma, S4317) were applied for 24hr to generate anterior foregut endoderm.

    Cell Culture:

    Article Title: Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
    Article Snippet: Generation and maintenance of murine iBCs Cell differentiation was performed in a complete serum free differentiation medium (cSFDM) consisting of a base medium of IMDM (ThermoFisher, 12440053) and Ham’s F12 (Cellgro, 10-080-CV) with B27 Supplement with retinoic acid (Invitrogen, 17504-44), N2 Supplement (Invitrogen, 17502-048), 0.1% bovine serum albumin Fraction V (Life Technologies, 15260-037), monothioglycerol (Sigma, M6145), Glutamax (Life Techologies, 35050-061), ascorbic acid (Sigma, A4544), and Primocin (Invivogen, ANTPM1). .. Directed differentiation was performed by adapting our previously described approach for deriving airway organoids from mouse PSC.32,35,36 Briefly, to induce definitive endoderm fate, EBs were dissociated into a single cell suspension and cultured in cSFDM supplemented with 50ng/mL recombinant human/mouse/rat Activin A (R&D systems, 338-AC) for 60hr allowing EBs to reform in suspension culture. .. Next, 100ng/mL recombinant mouse Noggin (R&D systems, 1967-NG) and 10mM SB431542 (Sigma, S4317) were applied for 24hr to generate anterior foregut endoderm.

    Concentration Assay:

    Article Title: Production method for nerve tissue
    Article Snippet: .. For example, Recombinant Human/Mouse/Rat Activin A (R&D systems, #338-AC) is added to a concentration of about 1 ng/ml to about 10 μg/ml, preferably about 10 ng/ml to about 1 μg/ml, more preferably about 100 ng/ml. ..

    Article Title: Production method for retinal tissue
    Article Snippet: .. For example, Recombinant Human/Mouse/Rat Activin A (R&D systems, #338-AC) is added to a concentration of about 1 ng/ml to about 10 μg/ml, preferably about 10 ng/ml to about 1 μg/ml, more preferably about 100 ng/ml. ..

    Article Title: Method for producing retinal tissue
    Article Snippet: .. For example, Recombinant Human/Mouse/Rat Activin A (R&D systems, #338-AC) is added to a concentration of about 1 ng/ml to about 10 μg/ml, preferably about 10 ng/ml to about 1 μg/ml, more preferably about 100 ng/ml. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Activin A, a Novel Chemokine, Induces Mouse NK Cell Migration via AKT and Calcium Signaling
    Article Snippet: .. Recombinant human/mouse/rat activin A (Cat# 338-AC), anti-human/mouse/rat activin A beta A subunit antibody (Cat# AF338) and enzyme-linked immunosorbent assay (ELISA) kit for activin A (Cat# DAC00B) were provided by R&D systems (Minneapolis, MN, USA). .. Recombinant murine CXCL12 (SDF-1α, Cat# 250-20A) and recombinant murine IL-2 (Cat# 212-12) were obtained from PeproTech (Rocky Hill, NJ, USA).

    other:

    Article Title: Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.
    Article Snippet: Next, 100ng/mL recombinant mouse Noggin (R&D systems, 1967-NG) and 10mM SB431542 (Sigma, S4317) were applied for 24hr to generate anterior foregut endoderm.



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